The retrogradely labeled cells were often concentrated in a particular distribution pattern in the dorsal horn. Retrogradely labeled cells were found in the dorsal horns of the cervical and lumbar segments of the spinal cord, and in both the ipsilateral and contralateral dorsal columns of the spinal cord. The spinal cord was then processed for immunocytochemical staining for HRP. The tracer HRP was injected into the dorsal root ganglia and surrounding tissues of the lumbar spine of the adult cat.

To examine the potential for retrograde transport by tracing neurons axons which enter the CNS through the subarachnoid space, we have used retrograde transneuronal transport of HRP to investigate the central connections of an identified peripheral spinal cord projection in the adult cat. game for PC on Linux.Retrograde axon traverses the midline via the central canal of the spinal cord in the adult cat.Īnterograde transport of horseradish peroxidase (HRP) is often used to identify synaptic sites of the central nervous system.

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